Recombinant Rat Pfn1 protein, His-tagged

• Cat.No.: Pfn1-56R
• Product Overview: Recombinant rat Pfn1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: Pfn1 also known as Profilin-1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein significantly enhances skin wound healing in-vitro and in-vivo that may be mediated by purinergic receptors. It is also active in endothelial cell migration and vessel sprouting. It is thought to regulate actin polymerization in response to extracellular signals.
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline(pH 7.4) containing 10% glycerol 1mM DTT
• Molecular Mass: 17.5 kDa (164aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAGWNA YIDSLMADGT CQDAAIVGYK DSPSVWAAVP GKTFVSITPA EVGVLVGKDR SSFFVNGLTL GGQKCSVIRD SLLQDGEFTM DLRTKSTGGA PTFNVTVTMT AKTLVLLMGK EGVHGGLINK KCYEMASHLR RSQY
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Pfn1 profilin 1 [ Rattus norvegicus ]
• Official Symbol: Pfn1
• Synonyms: PFN1; profilin 1; profilin-1; profilin I
• Gene ID: 64303
• mRNA Refseq: NM_022511
• Protein Refseq: NP_071956
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; G13 Signaling Pathway, organism-specific biosystem; Regulation of Actin Cytoskeleton, organism-specific biosystem; Regulation of actin cytoskeleton, organism-specific biosystem; Regulation of actin cytoskeleton, conserved biosystem; Salmonella infection, organism-specific biosystem
• Function: Rho GTPase binding; Rho GTPase binding; actin binding; actin binding; phosphatidylinositol-4,5-bisphosphate binding; proline-rich region binding; proline-rich region binding; protein binding; receptor binding