Recombinant Rat PRF1 Protein (25-554 aa), His-tagged

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Recombinant Rat PRF1 Protein (25-554 aa), His-tagged

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Cat.No. : PRF1-738R
Product Overview : Recombinant Rat PRF1 Protein (25-554 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Partial.
Description : Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the mbrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
Source : E. coli
Species : Rat
Tag : His
Form : Tris-based buffer, 50% glycerol
Molecular Mass : 62.9 kDa
Protein length : 25-554 aa
AA Sequence : ATRSECKQNH KFVPGVWAAG EGVDVTTLRR SSSFPVNTGK FLRPDRTCTL CKNALMNDGI QRLPVAIAHW RPHGSHCQRN VATTKVSSTE GVAREAAANI NNDWRAGLDV NPKPEANVHV SVAGSHSKIA NFAAEKAHQD QYNFNTDTVE CRMYSFRLAQ KPPLHPDFRK ALKNLPHNFN SSTEHAYRRL ISSYGTHFIT AVDLGGRVSV LTALRTCQLT LDGLTADEVG DCLSVEAQVS IGAQASVSSE YKACEEKKKQ HKIATSFHQT YRERHVEVLG GPLDSSNDLL FGNQATPEHF STWIASLPTR PDVVDYSLEP LHILLEDSDP KREALRQAIS HYVMSRARWR DCNRPCRAGQ HKSSRDSCQC VCQDSNVTNQ DCCPRQRGLA KLMVRNFQAK GLWGDYITST DAYLKVFFGG QEIRTGVVWN NNHPSWSDKM DFGNVLLSTG GPLRVQVWDA DNGWDDDLLG TCDKSPKSGF HEVNCPLNHG SIKFIYQANC LPDLTGETCL EYAPQGLLGD PRGNRSGAVW
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with concentration instruction is sent along with the products.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : Prf1 perforin 1 (pore forming protein) [ Rattus norvegicus ]
Official Symbol : PRF1
Synonyms : PRF1; perforin-1; P1; cytolysin; Cyta; RATCYTA; MGC108712;
Gene ID : 50669
mRNA Refseq : NM_017330
Protein Refseq : NP_059026
UniProt ID : P35763

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