Recombinant Rat PRF1 Protein (25-554 aa), His-tagged

• Cat.No.: PRF1-738R
• Product Overview: Recombinant Rat PRF1 Protein (25-554 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Partial.

Specification

• Description: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the mbrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 62.9 kDa
• Protein length: 25-554 aa
• AA Sequence: ATRSECKQNHKFVPGVWAAGEGVDV TTLRRSSSFPVNTGKFLRPDRTCTL CKNALMNDGIQRLPVAIAHWRPHGS HCQRNVATTKVSSTEGVAREAAANI NNDWRAGLDVNPKPEANVHVSVAGS HSKIANFAAEKAHQDQYNFNTDTVE CRMYSFRLAQKPPLHPDFRKALKNL PHNFNSSTEHAYRRLISSYGTHFIT AVDLGGRVSVLTALRTCQLTLDGLT ADEVGDCLSVEAQVSIGAQASVSSE YKACEEKKKQHKIATSFHQTYRERH VEVLGGPLDSSNDLLFGNQATPEHF STWIASLPTRPDVVDYSLEPLHILL EDSDPKREALRQAISHYVMSRARWR DCNRPCRAGQHKSSRDSCQCVCQDS NVTNQDCCPRQRGLAKLMVRNFQAK GLWGDYITSTDAYLKVFFGGQEIRT GVVWNNNHPSWSDKMDFGNVLLSTG GPLRVQVWDADNGWDDDLLGTCDKS PKSGFHEVNCPLNHGSIKFIYQANC LPDLTGETCLEYAPQGLLGDPRGNR SGAVW
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: Prf1 perforin 1 (pore forming protein) [ Rattus norvegicus ]
• Official Symbol: PRF1
• Synonyms: PRF1; perforin-1; P1; cytolysin; Cyta; RATCYTA; MGC108712
• Gene ID: 50669
• mRNA Refseq: NM_017330
• Protein Refseq: NP_059026
• UniProt ID: P35763

Reviews

07/04/2022

We observed remarkable consistency in product performance across multiple batches, reducing variability in our experiments.

11/14/2018

The manufacturer's history of producing high-quality products contributed to our trust in this protein.

05/11/2018

Collaboration with other research groups was made easier by the product's standardized use in the scientific community.

Q&As

What signaling pathways or stimuli influence the expression of perforin (PRF1) in immune cells?

Signaling pathways and stimuli such as T cell receptor (TCR) activation and exposure to infected cells influence the expression of perforin (PRF1) in immune cells.

In which immune cells is PRF1 expression particularly upregulated or modulated?

PRF1 expression is particularly upregulated or modulated in cytotoxic immune cells such as cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells.

Are there specific diseases or conditions associated with dysregulation or abnormal expression of PRF1?

Dysregulation or abnormal expression of PRF1 is associated with conditions like hemophagocytic lymphohistiocytosis (HLH) and certain autoimmune disorders.

How does perforin (PRF1) expression change under conditions of immune activation or response?

Perforin (PRF1) expression changes during immune activation, often being upregulated in response to various stimuli.

What factors influence the balance between upregulation and downregulation of PRF1 in immune cells?

The balance between upregulation and downregulation of PRF1 is influenced by various factors, including the type of immune response and the microenvironment.

What is the significance of altered PRF1 expression in the context of immune surveillance and cytotoxicity?

Altered PRF1 expression is significant for immune surveillance, as it plays a crucial role in the cytotoxicity of immune cells against infected or aberrant cells.

How does PRF1 contribute to immune responses against infected or aberrant cells, and what is its role in cytotoxicity?

PRF1 contributes to immune responses by enabling immune cells to induce apoptosis in target cells, contributing to cytotoxicity.