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Recombinant Rat Prf1 protein, His & T7-tagged

Cat.No. : Prf1-6804R
Product Overview : Recombinant Rat Prf1 aa. (Ala120~Asp353 (Accession # P35763)) fused with N-terminal His & T7 tag was produced in E. coli cells.
  • Specification
  • Gene Information
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Source : E. coli
Species : Rat
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 29.7kDa.
Protein length : Ala120~Asp353 (Accession # P35763)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 6.3.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name : Prf1 perforin 1 [ Rattus norvegicus (Norway rat) ]
Official Symbol : Prf1
Synonyms : Cyta; RATCYTA; P1; cytolysin; lymphocyte pore-forming protein; perforin 1 (pore forming protein); perforin-1
Gene ID : 50669
mRNA Refseq : NM_017330.2
Protein Refseq : NP_059026.2
UniProt ID : P35763

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What signaling pathways or stimuli influence the expression of perforin (PRF1) in immune cells? 07/09/2022

Signaling pathways and stimuli such as T cell receptor (TCR) activation and exposure to infected cells influence the expression of perforin (PRF1) in immune cells.

In which immune cells is PRF1 expression particularly upregulated or modulated? 04/23/2022

PRF1 expression is particularly upregulated or modulated in cytotoxic immune cells such as cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells.

Are there specific diseases or conditions associated with dysregulation or abnormal expression of PRF1? 08/19/2021

Dysregulation or abnormal expression of PRF1 is associated with conditions like hemophagocytic lymphohistiocytosis (HLH) and certain autoimmune disorders.

How does perforin (PRF1) expression change under conditions of immune activation or response? 05/05/2021

Perforin (PRF1) expression changes during immune activation, often being upregulated in response to various stimuli.

What factors influence the balance between upregulation and downregulation of PRF1 in immune cells? 09/04/2018

The balance between upregulation and downregulation of PRF1 is influenced by various factors, including the type of immune response and the microenvironment.

What is the significance of altered PRF1 expression in the context of immune surveillance and cytotoxicity? 10/14/2017

Altered PRF1 expression is significant for immune surveillance, as it plays a crucial role in the cytotoxicity of immune cells against infected or aberrant cells.

How does PRF1 contribute to immune responses against infected or aberrant cells, and what is its role in cytotoxicity? 04/29/2017

PRF1 contributes to immune responses by enabling immune cells to induce apoptosis in target cells, contributing to cytotoxicity.

Customer Reviews (3)

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Reviews
07/04/2022

    We observed remarkable consistency in product performance across multiple batches, reducing variability in our experiments.

    11/14/2018

      The manufacturer's history of producing high-quality products contributed to our trust in this protein.

      05/11/2018

        Collaboration with other research groups was made easier by the product's standardized use in the scientific community.

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