Recombinant Rat S100A1 Protein

• Cat.No.: S100A1-5210R
• Product Overview: Recombinant Rat S100A1 full length or partial length protein was expressed.

Specification

• Species: Rat
• Source: Mammalian Cells
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: S100a1 S100 calcium binding protein A1 [ Rattus norvegicus ]
• Official Symbol: S100A1
• Gene ID: 295214
• mRNA Refseq: NM_001007636.2
• Protein Refseq: NP_001007637.1
• UniProt ID: P35467

Citation

Bioactive nanoparticles improve calcium handling in failing cardiac myocytes

Journal: Nanomedicine    PubMed ID: 26223412    Data: 2015/11/1

Authors: Joshua T Maxwell, Inthirai Somasuntharam, Mary B Wagner

Article Snippet:Fluorescent Ca 2+ indicator dyes were purchased from Molecular Probes/Invitrogen (CA, USA).Fluorescent Ca 2+ indicator dyes were purchased from Molecular Probes/Invitrogen (CA, USA).. S100A1 recombinant protein was purchased from Creative BioMart (NY, USA), reconstituted in water at 0.5 μg/μl, and stored at -80°C until use.. Tyrode's solution contained (in mM) 138 NaCl, 4 KCl, 2 CaCl 2 , 1 MgCl 2 , 10 glucose and 10 HEPES; pH 7.4 with NaOH.Tyrode's solution contained (in mM) 138 NaCl, 4 KCl, 2 CaCl 2 , 1 MgCl 2 , 10 glucose and 10 HEPES; pH 7.4 with NaOH.

(A) Representative confocal line scans and the corresponding fluorescence profiles (ΔF/F0) of Ca2+ sparks from permeabilized rat myocytes from CTL, PAB or PAB treated with de-encapsulated S100A1 (PAB + S100A1). Fluorescent profiles were generated from the line scan regions marked by black boxes. (B–D) Summary data of the spark amplitude (B), spark frequency (C), full width at half maximum (D) and full duration at half maximum (E) measured from the three groups. CTL: n = 80 sparks from n = 7 rats; PAB: n = 212 sparks from n = 10 rats; PAB + S100A1: n = 33 sparks from n = 9 rats.

(A) Representative line scans and the corresponding fluorescence profiles (ΔF/F0) of Ca2+ sparks from intact rat myocytes from PAB or PAB treated with empty GlcNAc nanoparticles (empty-GlcNAc), S100A1-loaded GlcNAc nanoparticles (S100A1-GlcNAc) or external S100A1 native protein. Fluorescent profiles were generated from the line scan regions marked by black boxes. (B–D) Summary data of the spark amplitude (B), spark frequency (C), FWHM; (D) and FDHM; (E) measured from the four groups. PAB: n = 176 sparks from n = 9 rats; Empty-GlcNAc: n = 119 sparks from n = 10 rats; S100A1-GlcNAc: n = 145 sparks from n = 11 rats; S100A1 protein: n = 39 sparks from n = 3 rats.

(A) Representative sarcomere shortening, (B) Ca2+ transient amplitude and (C) diastolic Ca2+ traces from PAB, empty-GlcNAc treated and S100A1-GlcNAc treated myocytes during 0.5 and 1 Hz electrical stimulation. Summary bar graphs are shown for each parameter below the traces. Sarcomere shortening is shown as the percentage change in sarcomere length during contraction. Ca2+ transient amplitude (ΔR) and diastolic Ca2+ (R) measurements were made in cells loaded with 10 μM of the ratiometric Ca2+ indicator fura-2/AM. Representative traces are from cells during (A & B) 1.0 Hz or (C) 0.5 Hz stimulation. PAB: n = 8–13 cells from n = 6 rats; empty-GlcNAc: n = 9–13 cells from n = 6 rats; S100A1-GlcNAc: n = 8–12 cells from n = 6 rats.