Active Recombinant Rat Tnf protein, His-tagged

• Cat.No.: TNF-6193R
• Product Overview: Recombinant Rat Tnf protein(Leu80-Leu235), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 80-235 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Recombinant Rat TNF-alpha induces cytotoxicity in the L-929 Mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3.37-13.48 pg/mL, corresponding to a specific activity of 7.42×10^7~2.97×10^8 units/mg.
• Molecular Mass: 18.91 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Tnf tumor necrosis factor [ Rattus norvegicus ]
• Official Symbol: Tnf
• Synonyms: TNF; tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2; Tnfa; RATTNF; TNF-alpha; MGC124630
• Gene ID: 24835
• mRNA Refseq: NM_012675
• Protein Refseq: NP_036807