Recombinant Rhesus CXCL13 protein

• Cat.No.: CXCL13-1322R
• Product Overview: Recombinant Rhesus CXCL13 protein was expressed in Escherichia coli.

Specification

• Description: B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches.
• Source: E.coli
• Species: Rhesus
• Form: Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris-HCl, pH 8.0, 300mM NaCl.
• Molecular Mass: Approximately 10.3 kDa, a single non-glycosylated polypeptide chain containing 87 amino acids.
• Protein length: 87
• AA Sequence: VLEVYYTHLRCRCVQESSVFIPRRF IDRIQISPRGNGCPRKEIIVWKKNK SVVCVDPQAEWIQRIMEMLRKKSSS TPPVPVFKRKIP
• Endotoxin: Less than 0.01 EU/μg of rRhCXCL-13 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
• Tag: Non

Gene Information

• Gene Name: CXCL13
• Official Symbol: CXCL13
• Gene ID: 574224
• mRNA Refseq: NM_001032880.1
• Protein Refseq: NP_001028052.1
• UniProt ID: Q8HYN9

Reviews

07/10/2021

Stability of CXCL13 was very good during long-term storage, and its properties remained unchanged after prolonged storage, indicating its excellent storage stability.

06/21/2021

The broad storage conditions of proteins make the experimental process more stable and reliable, which provides strong support for scientific research.

08/14/2019

In the process of CXCL13 production, it can be seen that it pays great attention to purity and biological activity, which makes its product extremely reliable.

Q&As

Is the expression pattern of CXCL13 protein related to the clinical prognosis of specific diseases?

Expression pattern of CXCL13 protein may be related to the clinical prognosis of specific diseases, but the specific association needs further research and verification.

What are the expression characteristics of CXCL13 protein in immune cells?

The protein is mainly expressed in immune cells by activated dendritic cells, macrophages and inflammatory cells.

Is there a regulatory mechanism for CXCL13 protein expression during inflammation?

The expression of CXCL13 protein is regulated by a variety of signaling pathways, including NF-κB, AP-1, etc. Stimulation of inflammatory factors and cytokines may lead to upregulation of CXCL13 expression.

What is the role of CXCL13 protein in lymph node metastasis?

In lymph node metastasis, the CXCL13 protein promotes the localization and metastasis of cancer cells by attracting CXCR5-positive cancer cells or immune cells.

What are the implications of CXCL13 protein research for new drug development?

CXCL13 protein plays an important role in immune regulation and inflammatory response, and studying the function and regulatory mechanism of CXCL13 protein will help the development of new drugs, such as targeted drugs targeting CXCL13 and its related signaling pathways, which may be applied to the treatment of related diseases.

What are the potential applications of CXCL13 protein in immunotherapy?

The CXCL13 protein is involved in immunomodulation, and its bound antibodies or specific receptor antagonists may contribute to the design and development of immunotherapy.