Active Recombinant Rhesus / Cynomolgus IL18 protein, Avi-tagged, Biotinylated

• Cat.No.: IL18-385R
• Product Overview: Biotinylated Recombinant Rhesus / Cynomolgus IL18 protein (A0A2K5TJ84)(Tyr37-Asp193) is expressed from E.coli with Avi tag at the C-terminus.

Specification

• Species: Rhesus / Cynomolgus
• Source: E.coli
• Tag: Avi
• Protein Length: 37-193 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Cynomolgus IL-18BP, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus IL-18, Avi Tag with the EC50 of 3.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 20.34 kDa same as Bis-Tris PAGE result.
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Official Symbol: IL18

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.