Description : |
B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. |
Source : |
E.coli |
Species : |
Rhesus |
Form : |
Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris-HCl, pH 8.0, 300mM NaCl. |
Molecular Mass : |
Approximately 10.3 kDa, a single non-glycosylated polypeptide chain containing 87 amino acids. |
Protein length : |
87 |
AA Sequence : |
VLEVYYTHLRCRCVQESSVFIPRRF IDRIQISPRGNGCPRKEIIVWKKNK SVVCVDPQAEWIQRIMEMLRKKSSS TPPVPVFKRKIP |
Endotoxin : |
Less than 0.01 EU/μg of rRhCXCL-13 as determined by LAL method. |
Purity : |
>95% by SDS-PAGE and HPLC analysis. |
Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |