Recombinant SARS-CoV-2 S Protein, His-tagged

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Recombinant SARS-CoV-2 S Protein, His-tagged

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Cat.No. : S-005S
Product Overview : Recombinant SARS-CoV-2 Spike protein (RBD) with a His tag was expressed in Human Cells.
Description : SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. It is believed that SARS-CoV-2 Spike Protein (RBD) has potential value for the diagnosis of the virus.
Source : Human Cells
Species : SARS-CoV-2
Tag : His
Form : Liquid
Bio-activity : SARS-CoV-2 Spike protein (RBD, His Tag) can bind with Human ACE2 in functional ELISA assay.
Molecular Mass : 30 KD
Purity : > 90% as analyzed by SDS-PAGE
Storage : The product can be stored at -20 centigrade or below. Avoid repeated freezing and thawing cycles. The shelf life of the product is unspecified.
Storage Buffer : PBS, pH 7.2
Figure :

Immobilized ACE-2 Fc Chimera, Human at 2 μg/mL can bind SARS-CoV-2 Spike protein (RBD, His Tag) with a serial dilution. THETM His Tag Antibody [HRP], mAb, Mouse is used as a secondary antibody (0.2 μg/mL).

Western blot analysis of SARS-CoV-2 Spike protein (RBD, His Tag) using S1 Antibody :

Lane 1: 10 ng of SARS-CoV-2 Spike protein (RBD, His Tag)
Lane 2: 5 ng of SARS-CoV-2 Spike protein (RBD, His Tag)

Western blot analysis of SARS-CoV-2 Spike protein (RBD, His Tag) using His Antibody. :

Lane 1: 10 ng of SARS-CoV-2 Spike protein (RBD, His Tag)
Lane 2: 5 ng of SARS-CoV-2 Spike protein (RBD, His Tag)

SDS-PAGE :

1: 2 μg of RBD, reducing (R)
2: 2 μg of RBD, non-reducing (N)

Gene Name : S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
Official Symbol : S
Synonyms : S; surface glycoprotein; spike glycoprotein; surface glycoprotein
Gene ID : 43740568
UniProt ID : P0DTC2

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