| Species : |
Human |
| Source : |
Yeast |
| Description : |
This gene is a member of the IL-17 receptor family which includes five members (IL-17RA-E) and the encoded protein is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. The encoded protein elicits crucial impacts on host defense, cell trafficking, immune modulation, and tissue repair, with a key role in the induction of innate immune defenses. This cytokine stimulates non-hematopoietic cells and promotes chemokine production thereby attracting myeloid cells to inflammatory sites. This cytokine also regulates the activities of NF-kappaB and mitogen-activated protein kinases and can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). IL-17A plays a pivotal role in various infectious diseases, inflammatory and autoimmune disorders, and cancer. High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The lung damage induced by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL17A. |
| Form : |
Sterile lyophilized powder |
| Molecular Mass : |
15-22 kDa (Monomer) |
| Bio-activity : |
Recombinant human IL-17A-induced IL-6 production in WISH cells is quantified using an IL-6 ELISA kit. |
| Endotoxin : |
<1 EU/μg determined by LAL. |
| Purity : |
≥97% |
| Storage : |
Lyophilized samples remain stable for 36 months from the date of manufacture when stored at –20 to –70 centigrade. After reconstitution, the cytokine can be kept for one month at 2–8 centigrade under sterile conditions or for three months at –20 to –70 centigrade in a manual-defrost freezer, without significant loss of activity. Avoid repeated freeze–thaw cycles. |
| Storage Buffer : |
This product is supplied as a sterile lyophilized powder in a buffer of 100 mM Gly, 100 mM NaCl, pH 3.0. |
| Reconstitution : |
Before opening the vial, centrifuge at 10, 000 rpm for 30–60 seconds, or at 3, 000 rpm for 5 minutes, then reconstitute with 0.2 mL of sterile water. |
