Active GMP Recombinant Human IL17A protein
Cat.No. : | IL17A-4331HG |
Product Overview : | Recombinant Human IL17A was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines. |
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Description : | Human Interleukin-17A (IL-17A) is encoded by the IL17A gene located on the chromosome 6 and belongs to the IL-17 family that contains IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. They have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, but no sequence similarity to any other known cytokines. Interleukin 17 is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Mature IL-17 containing one potential N-linked glycosylation site. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, IL-17 exhibits 63 % amino acid identity with mouse IL-17. High levels of human IL-17 were induced from primary peripheral blood CD4+ T cells upon stimulation and they can induce stromal cells to produce proinflammatory and hematopoietic cytokines. |
Source : | E. coli |
Species : | Human |
Form : | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 7.5 ng/ml, corresponding to a specific activity of > 1.3 × 10^5 IU/mg. |
Molecular Mass : | Approximately 31.0 kDa, a disulfide-linked homodimer of two 132 amino acid polypeptide chains. |
AA Sequence : | GITIPRNPGCPNSEDKNFPRTVMVN LNIHNRNTNTNPKRSSDYYNRSTSP WNLHRNEDPERYPSVIWEAKCRHLG CINADGNVDYHMNSVPIQQEILVLR REPPHCPNSFRLEKILVSVGCTCVT PIVHHVA |
Endotoxin : | Less than 1 EU/µg of rHuIL-17 as determined by LAL method. |
Purity : | > 95 % by SDS-PAGE and HPLC analyses. |
Storage : | This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles. |
Reconstitution : | Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Quality Statement : | Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Tag : | Non |
Gene Name : | IL17A interleukin 17A [ Homo sapiens ] |
Official Symbol : | IL17A |
Synonyms : | IL17; CTLA8; IL-17; IL-17A; interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8) |
Gene ID : | 3605 |
mRNA Refseq : | NM_002190 |
Protein Refseq : | NP_002181 |
MIM : | 603149 |
UniProt ID : | Q16552 |
Products Types
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IL17A-848M | Recombinant Mouse IL17A Protein (Ala26-Ala158) | +Inquiry |
Il17a-093M | Active Recombinant Mouse Il17a Protein | +Inquiry |
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◆ Lysates | ||
IL17A-001HCL | Recombinant Human IL17A cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewGood for receptor studies.
Effective in Th17 differentiation.
High bioactivity in various tissue types.
Q&As (10)
Ask a questionAnimal models, such as experimental autoimmune encephalomyelitis, explore IL17A's role in autoimmunity.
Inducing IL17A expression in T cells involves culturing them with specific cytokines.
Studying IL17A's influence on tissue-specific inflammation in humans involves analyzing patient samples.
Neutralizing antibodies block IL17A's effects in cell cultures or animal models.
Multiplex cytokine assays use fluorescent beads to measure IL17A and other cytokines simultaneously.
IL17RA signaling is triggered by IL17A binding and is studied through downstream phosphorylation assays.
Methods like transepithelial electrical resistance (TEER) assay evaluate IL17A's effect on barrier function.
Inflammatory models, like airway inflammation assays, examine IL17A's impact on neutrophil recruitment.
Flow cytometry or intracellular staining help identify IL17A-producing cell subsets within tissues.
Experimental designs combine cancer cell lines with IL17A stimulation to investigate its pro-tumor effects.
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