Active Recombinant Human ALPL protein, His-tagged

• Cat.No.: ALPL-386H
• Product Overview: Recombinant Human ALPL protein(Leu18-Ala500), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 18-500 aa
• Form: Lyophilized from a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, 2mM MgSO4, 0.1mM ZnCl2,pH 7.5.
• Bio-activity: Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >29519.5 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: 54.04 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: ALPL alkaline phosphatase, liver/bone/kidney [ Homo sapiens ]
• Official Symbol: ALPL
• Synonyms: ALPL; alkaline phosphatase, liver/bone/kidney; HOPS; alkaline phosphatase, tissue-nonspecific isozyme; TNSALP; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme; TNAP; APTNAP; AP-TNAP; FLJ40094; FLJ93059; MGC161443; MGC167935
• Gene ID: 249
• mRNA Refseq: NM_000478
• Protein Refseq: NP_000469
• MIM: 171760
• UniProt ID: P05186