Active Recombinant Canine IL2RA, His-tagged

• Cat.No.: IL2RA-460C
• Product Overview: Recombinant Canine IL2RA (Accession # BAI49682) Met1-Ile238, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Canine
• Tag: His
• Predicted N Terminal: Tyr19 & Thr21
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to block IL-2-induced proliferation of CTLL-2 mouse cytotoxic T cells.The ED50 for this effect is typically 0.4-2.4 µg/mL in the presence of 1 ng/mL of Recombinant Canine IL-2 (Cys147Ser).
• Molecular Mass: Recombinant Canine IL2RA, His-tagged has a calculated MW of 25.7 kDa (monomer). In SDS-PAGE migrates as 40-55 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: IL2RA interleukin 2 receptor, alpha [ Canis lupus familiaris (dog) ]
• Official Symbol: IL2RA
• Synonyms: IL2RA; interleukin 2 receptor, alpha; CD25; IL-2RA; interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; interleukin 2 receptor alpha chain; interleukin-2 receptor alpha subunit; p55
• Gene ID: 403870
• mRNA Refseq: NM_001003211
• Protein Refseq: NP_001003211
• UniProt ID: O62802
• Chromosome Location: 2