IL33-1034C
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant mature form of canine IL33 (O97863) (Ser 110-Ser 263) was expressed, with an initial Met at the N-terminus.
Source :
E. coli
Species :
Canine
Tag :
N/A
Form :
Lyophilized from sterile PBS, pH7.4
Bio-activity :
Measured by its binding ability in a functional ELISA. Immobilized Canine IL33 at 10 μg/ml (100 μl/well) can bind human IL1R4-Fc, The EC50 of human IL1R4-Fc is 0.25-0.66 μg/ml.
Molecular Mass :
The recombinant canine IL33 consists of 155 amino acids and has a calculated molecular mass of 17.8 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 19KDa band.
Protein Length :
Ser 110-Ser 263
Endotoxin :
< 1.0 EU per μg of the protein as determined by the LAL method
Purity :
> 97 % as determined by SDS-PAGE
Storage :
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution :
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.