Recombinant Human Interleukin 10, His-tagged

• Cat.No.: IL10-530H
• Product Overview: Recombinant IL10 protein, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Interleukin-10 (IL 10) is also known as human cytokine synthesis inhibitory factor (CSIF) and inhibits the synthesis of pro-inflammatory cytokines like IFN-gamma, IL2, IL3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. This protein has effects in immunoregulation and inflammation and down-regulates the expression of Th1 cytokines, MHC class II proteins, and costimulatory molecules on macrophages.
• Concentration: 0.5 mg/ml
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol.
• Purity: > 95% by SDS - PAGE
• Sequence: 19-178 amino acids: MGSSHHHHHH SSGLVPRGSH MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
• Molecular Mass: 20.94 kDa (181 aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Endotoxin Levels: < 1.0 EU per ug of protein (determined by LAL method)
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: IL10 interleukin 10 [ Homo sapiens ]
• Official Symbol: IL10
• Synonyms: CSIF; IL10A; MGC1264 50; MGC126451; TGIF; B-TCGF; IL-10; Cytokine synthesis inhibitory factor; Interleukin-10 precursor; IL10; interleukin 10
• Gene ID: 3586
• mRNA Refseq: NM_000572
• Protein Refseq: NP_000563
• MIM: 124092
• UniProt ID: P22301
• Chromosome Location: 1q31-q32
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; T cell receptor signaling pathway
• Function: cytokine activity; interleukin-10 receptor binding; protein binding