Species : |
Equine |
Source : |
E.coli |
Protein Length : |
21-149 a.a. |
Predicted N Terminal : |
Met & Ala21 |
Form : |
Lyophilized from a 0.2 µm filtered solution in Sodium Acetate with BSA as a carrier protein. |
Bio-activity : |
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295.The ED50 for this effect is typically 0.3 - 1.5 µg/mL. |
Molecular Mass : |
Recombinant Equine IL2, Met-tagged has a calculated MW of 15 kDa. |
Purity : |
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : |
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Reconstitution : |
Reconstitute at 200 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |