Active Recombinant Human IL2 Protein (Ala21-Thr153), C-His tagged, Animal-free, Carrier-free

• Cat.No.: IL2-165H
• Product Overview: Recombinant Human IL2 Protein (Ala21-Thr153) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ala21-Thr153
• Description: Interleukin-2 (IL-2) is a 15.5 kDa four bundle, immuno-modulatory cytokine which is expressed predominately by antigen-simulated CD4+ T cells, CD8+ T cells, natural killer (NK) cells, and dendritic cells (DC). IL-2 plays a fundamental role in promoting NK cell proliferation, cytotoxicity, and B cells differentiation. IL-2 modulate T cell differentiation programs, promoting naïve CD4+ T cell differentiation into T helper-1 (Th1) and T helper-2 (Th2) cells.
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is < 3 ng/mL. The specific activity of recombinant human IL-2 is >5 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 16.4 kDa.
  The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.01 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens (human) ]
• Official Symbol: IL2
• Synonyms: IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; involved in regulation of T-cell clonal expansion; IL-2; lymphokine
• Gene ID: 3558
• mRNA Refseq: NM_000586
• Protein Refseq: NP_000577
• MIM: 147680
• UniProt ID: P60568