| Species : |
Equine |
| Source : |
Yeast |
| Tag : |
Non |
| Description : |
Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. In terms of host response to a foreign pathogen, IL-6 has been shown, in mice, to be required for resistance against the bacterium, Streptococcus pneumoniae. IL-6 is also a "myokine," a cytokine produced from muscle, and is elevated in response to muscle contraction. |
| Formulation/ Reconstitution : |
Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein. |
| Purification : |
Ion-exchange chromatography. |
| Purity : |
>95% as visualized by SDS-PAGE analysis. |
| Amino Acid Sequence : |
TPLPLGEDET TSNGPLLTTA DKTKQHIKYI LGKISALKNE MCNNFSKCEN SKEVLAENNL NLPKMAEKDG CFQSGFNQET CLMKITTGLS EFQIYLEYLQ NEFKGEKENI KTMQISTKVL VQILMQKMKN PEVTTPDPTA KSSLLAKLHS QNEWLKNTTT HLILRSLEDF LQFSLRAVRI M. |
| Bioactivity : |
The biological activity of recombinant equine IL-6 was measured in a cell proliferation assay using the mouse B9 cell line. The ED50 for this effect is typically0.05 – 1.0 ng/mL. |
| Stability And Storage : |
Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles. |
