Species : |
Feline |
Source : |
Mammalian Cells |
Tag : |
His |
Protein Length : |
27-185 a.a. |
Predicted N Terminal : |
Ser27 |
Form : |
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein. |
Bio-activity : |
Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3.The ED50 for this effect is typically 0.5 - 2 ng/mL. |
Molecular Mass : |
Recombinant Feline FLT3LG, His-tagged has a calculated MW of 18.5 kDa. In SDS-PAGE migrates as 25-33 kDa, reducing conditions. |
Purity : |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : |
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Reconstitution : |
Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |