Active Recombinant Full Length Human IRF5 Protein, C-Flag-tagged

• Cat.No.: IRF5-555HFL
• Product Overview: Recombinant Full Length Human IRF5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Cell treatment
• Molecular Mass: 56.5 kDa
• AA Sequence: MNQSIPVAPTPPRRVRLKPWLVAQV NSCQYPGLQWVNGEKKLFCIPWRHA TRHGPSQDGDNTIFKAWAKE TGKYTEGVDEADPAKWKANLRCALN KSRDFRLIYDGPRDMPPQPYKIYEV CSNGPAPTDSQPPEDYSFGA GEEEEEEEELQRMLPSLSLTDAVQS GPHMTPYSLLKEDVKWPPTLQPPTL QPPVVLGPPAPDPSPLAPPP GNPAGFRELLSEVLEPGPLPASLPP AGEQLLPDLLISPHMLPLTDLEIKF QYRGRPPRALTISNPHGCRL FYSQLEATQEQVELFGPISLEQVRF PSPEDIPSDKQRFYTNQLLDVLDRG LILQLQGQDLYAIRLCQCKV FWSGPCASAHDSCPNPIQREVKTKL FSLEHFLNELILFQKGQTNTPPPFE IFFCFGEEWPDRKPREKKLI TVQVVPVAARLLLEMFSGELSWSAD SIRLQISNPDLKDRMVEQFKELHHI WQSQQRLQPVAQAPPGAGLG
  VGQGPWPMHPAGMQTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transcription Factors
• Protein Pathways: Toll-like receptor signaling pathway

Gene Information

• Gene Name: IRF5 interferon regulatory factor 5 [ Homo sapiens (human) ]
• Official Symbol: IRF5
• Synonyms: SLEB10
• Gene ID: 3663
• mRNA Refseq: NM_001098627.4
• Protein Refseq: NP_001092097.2
• MIM: 607218
• UniProt ID: Q13568