Active Recombinant Full Length Human ITGAL Protein, C-Flag-tagged

• Cat.No.: ITGAL-168HFL
• Product Overview: Recombinant Full Length Human ITGAL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Far-Western blot
• Molecular Mass: 126.1 kDa
• AA Sequence: MKDSCITVMAMALLSGFFFFAPASS YNLDVRGARSFSPPRAGRHFGYRVL QVGNGVIVGAPGEGNSTGSL YQCQSGTGHCLPVTLRGSNYTSKYL GMTLATDPTDGSILACDPGLSRTCD QNTYLSGLCYLFRQNLQGPM LQGRPGFQECIKGNVDLVFLFDGSM SLQPDEFQKILDFMKDVMKKLSNTS YQFAAVQFSTSYKTEFDFSD YVKRKDPDALLKHVKHMLLLTNTFG AINYVATEVFREELGARPDATKVLI IITDGEATDSGNIDAAKDII RYIIGIGKHFQTKESQETLHKFASK PASEFVKILDTFEKLKDLFTELQKK IYVIEGTSKQDLTSFNMELS SSGISADLSRGHAVVGAVGAKDWAG GFLDLKADLQDDTFIGNEPLTPEVR AGYLGYTVTWLPSRQKTSLL ASGAPRYQHMGRVLLFQEPQGGGHW SQVQTIHGTQIGSYFGGELCGVDVD QDGETELLLIGAPLFYGEQR GGRVFIYQRRQLGFEEVSELQGDPG YPLGRFGEAITALTDINGDGLVDVA VGAPLEEQGAVYIFNGRHGG LSPQPSQRIEGTQVLSGIQWFGRSI HGVKDLEGDGLADVAVGAESQMIVL SSRPVVDMVTLMSFSPAEIP VHEVECSYSTSNKMKEGVNITICFQ IKSLIPQFQGRLVANLTYTLQLDGH RTRRRGLFPGGRHELRRNIA VTTSMSCTDFSFHFPVCVQDLISPI NVSLNFSLWEEEGTPRDQRAQGKDI PPILRPSLHSETWEIPFEKN CGEDKKCEANLRVSFSPARSRALRL TAFASLSVELSLSNLEEDAYWVQLD LHFPPGLSFRKVEMLKPHSQ IPVSCEELPEESRLLSRALSCNVSS PIFKAGHSVALQMMFNTLVNSSWGD SVELHANVTCNNEDSDLLED NSATTIIPILYPINILIQDQEDSTL YVSFTPKGPKIHQVKHMYQVRIQPS IHDHNIPTLEAVVGVPQPPS EGPITHQWSVQMEPPVPCHYEDLER LPDAAEPCLPGALFRCPVVFRQEIL VQVIGTLELVGEIEASSMFS LCSSLSISFNSSKHFHLYGSNASLA QVVMKVDVVYEKQMLYLYVLSGIGG LLLLLLIFIVLYKVGFFKRN
  LKEKMEAGRGVPNGIPAEDSEQLAS GQEAGDPGCLKPLHEKDSESGGGKD TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, Viral myocarditis

Gene Information

• Gene Name: ITGAL integrin subunit alpha L [ Homo sapiens (human) ]
• Official Symbol: ITGAL
• Synonyms: CD11A; LFA-1; LFA1A
• Gene ID: 3683
• mRNA Refseq: NM_002209.3
• Protein Refseq: NP_002200.2
• MIM: 153370
• UniProt ID: P20701