Active Recombinant Full Length Human NEU1 Protein, C-Flag-tagged

• Cat.No.: NEU1-178HFL
• Product Overview: Recombinant Full Length Human NEU1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Cell treatment
• Molecular Mass: 40.2 kDa
• AA Sequence: MTGERPSTALPDRRWGPRILGFWGG CRVWVFAAIFLLLSLAASWSKAEND FGLVQPLVTMEQLLWVSGRQ IGSVDTFRIPLITATPRGTLLAFAE ARKMSSSDEGAKFIALRRSMDQGST WSPTAFIVNDGDVPDGLNLG AVVSDVETGVVFLFYSLCAHKAGCQ VASTMLVWSKDDGVSWSTPRNLSLD IGTEVFAPGPGSGIQKQREP RKGRLIVCGHGTLERDGVFCLLSDD HGASWRYGSGVSGIPYGQPKQENDF NPDECQPYELPDGSVVINAR NQNNYHCHCRIVLRSYDACDTLRPR DVTFDPELVDPVVAAGAVVTSSGIV FFSNPAHPEFRVNLTLRWSF SNGTSWRKETVQLWPGPSGYSSLAT LEGSMDGEEQAPQLYVLYEKGRNHY TESISVAKISVYGTLTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome, Other glycan degradation, Sphingolipid metabolism

Gene Information

• Gene Name: NEU1 neuraminidase 1 [ Homo sapiens (human) ]
• Official Symbol: NEU1
• Synonyms: NEU; NANH; SIAL1
• Gene ID: 4758
• mRNA Refseq: NM_000434.4
• Protein Refseq: NP_000425.1
• MIM: 608272
• UniProt ID: Q99519