Active Recombinant Full Length Human PIK3CA Protein, GST-tagged

Cat.No. : PIK3CA-7972HF
Product Overview : Full-length human PIK3CA[1-1068(end) amino acids of accession number NP_006209.2] was co- expressed as N-terminal GST-fusion protein (151 kDa) with PIK3R1[1-724(end) amino acids of accession number NP_852664.1] using baculovirus expression system. GST-PIK3CA/PIK3R1 was purified by using glutathione sepharose chromatography.
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Source : Insect (sf21)
Species : Human
Tag : GST
Form : 50 mM Tris-HCl, 150 mM NaCl, 0.01% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Bio-activity : The activity was determined by ADP-Glo™ Assay. The enzyme was incubated with Lipid substrate and Mg(or Mn)/ATP. The phosphorylation was detected by the ADP-Glo™ Kinase Assay technology (luminescent ADP detection assay).
Protein length : 1-1068(end)
Purity : 90%
Applications : SDS-PAGE
Storage : Store at -80C. Avoid repeating freeze-thaws.
SDS-PAGE: The purity was assessed by SDS-PAGE/CBB staining.
Gene Name : phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Official Symbol : PIK3CA
Synonyms : PIK3CA;CLAPO;CLOVE;CWS5;MCAP;MCM;MCMTC;PI3K;PI3K-alpha;p110-alpha
Protein Refseq : NP_006209.2
UniProt ID : P42336

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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