Active Recombinant Full Length Human PNP Protein, C-Flag-tagged

• Cat.No.: PNP-715HFL
• Product Overview: Recombinant Full Length Human PNP Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: WB standard
• Molecular Mass: 31.9 kDa
• AA Sequence: MENGYTYEDYKNTAEWLLSHTKHRP QVAIICGSGLGGLTDKLTQAQIFDY GEIPNFPRSTVPGHAGRLVF GFLNGRACVMMQGRFHMYEGYPLWK VTFPVRVFHLLGVDTLVVTNAAGGL NPKFEVGDIMLIRDHINLPG FSGQNPLRGPNDERFGDRFPAMSDA YDRTMRQRALSTWKQMGEQRELQEG TYVMVAGPSFETVAECRVLQ KLGADAVGMSTVPEVIVARHCGLRV FGFSLITNKVIMDYESLEKANHEEV LAAGKQAAQKLEQFVSILMA
  SIPLPDKASTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism

Gene Information

• Gene Name: PNP purine nucleoside phosphorylase [ Homo sapiens (human) ]
• Official Symbol: PNP
• Synonyms: NP; PUNP; PRO1837
• Gene ID: 4860
• mRNA Refseq: NM_000270.4
• Protein Refseq: NP_000261.2
• MIM: 164050
• UniProt ID: P00491