Active Recombinant Full Length Human PPM1G Protein, C-Flag-tagged

• Cat.No.: PPM1G-560HFL
• Product Overview: Recombinant Full Length Human PPM1G Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: In vitro phosphatase assay
• Molecular Mass: 59.1 kDa
• AA Sequence: MGAYLSQPNTVKCSGDGVGAPRLPL PYGFSAMQGWRVSMEDAHNCIPELD SETAMFSVYDGHGGEEVALY CAKYLPDIIKDQKAYKEGKLQKALE DAFLAIDAKLTTEEVIKELAQIAGR PTEDEDEKEKVADEDDVDNE EAALLHEEATMTIEELLTRYGQNCH KGPPHSKSGGGTGEEPGSQGLNGEA GPEDSTRETPSQENGPTAKA YTGFSSNSERGTEAGQVGEPGIPTG EAGPSCSSASDKLPRVAKSKFFEDS EDESDEAEEEEEDSEECSEE EDGYSSEEAENEEDEDDTEEAEEDD EEEEEEMMVPGMEGKEEPGSDSGTT AVVALIRGKQLIVANAGDSR CVVSEAGKALDMSYDHKPEDEVELA RIKNAGGKVTMDGRVNGGLNLSRAI GDHFYKRNKNLPPEEQMISA LPDIKVLTLTDDHEFMVIACDGIWN VMSSQEVVDFIQSKISQRDENGELR LLSSIVEELLDQCLAPDTSG
  DGTGCDNMTCIIICFKPRNTAELQP ESGKRKLEEVLSTEGAEENGNSDKK KKAKRDTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Phosphatase

Gene Information

• Gene Name: PPM1G protein phosphatase, Mg2+/Mn2+ dependent 1G [ Homo sapiens (human) ]
• Official Symbol: PPM1G
• Synonyms: PP2CG; PPP2CG; PP2CGAMMA
• Gene ID: 5496
• mRNA Refseq: NM_177983.3
• Protein Refseq: NP_817092.1
• MIM: 605119
• UniProt ID: O15355