Active Recombinant Full Length Human VHL Protein, C-Flag-tagged

• Cat.No.: VHL-259HFL
• Product Overview: Recombinant Full Length Human VHL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Binding assay
• Molecular Mass: 24 kDa
• AA Sequence: MPRRAENWDEAEVGAEEAGVEEYGP EEDGGEESGAEESGPEESGPEELGA EEEMEAGRPRPVLRSVNSRE PSQVIFCNRSPRVVLPVWLNFDGEP QPYPTLPPGTGRRIHSYRGHLWLFR DAGTHDGLLVNQTELFVPSL NVDGQPIFANITLPVYTLKERCLQV VRSLVKPENYRRLDIVRSLYEDLED HPNVQKDLERLTQERIAHQR
  MGDTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis

Gene Information

• Gene Name: VHL von Hippel-Lindau tumor suppressor [ Homo sapiens (human) ]
• Official Symbol: VHL
• Synonyms: RCA1; VHL1; pVHL; HRCA1
• Gene ID: 7428
• mRNA Refseq: NM_000551.4
• Protein Refseq: NP_000542.1
• MIM: 608537
• UniProt ID: P40337