Active Recombinant Horse IL1b protein, His-tagged

• Cat.No.: IL1b-5641H
• Product Overview: Active Recombinant Horse IL1b protein(Q28386)(63~247 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Horse
• Source: E.coli
• Tag: His
• Protein Length: 63-247 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Interleukin 1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1β can induced IL-8 production in A549 cells. To test the bioactivity of IL-1β, A549 cells were seeded into 24-well plate at a density of 1x10^5 cells/mL, and allowed to attach overnight before treated with certain concentrations of IL-1β for 24h and IL-8 levels in the cell supernatant were determined by ELISA.
• Molecular Mass: 22.6kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: IL1B interleukin 1, beta [ Equus caballus ]
• Official Symbol: IL1b
• Synonyms: IL1B; interleukin 1, beta; interleukin-1 beta; IL-1 beta; IL-1B
• Gene ID: 100034237
• mRNA Refseq: NM_001082526
• Protein Refseq: NP_001075995