Active Recombinant Human BTLA Protein, Fc-Avi-tagged, Biotinylated
Cat.No. : | BTLA-567H |
Product Overview : | Biotinylated Human BTLA (31-150), Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag. |
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Source : | HEK293 |
Species : | Human |
Tag : | Fc-Avi |
Predicted N Terminal : | Lys 31 |
Form : | Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arg, 150 mM NaCl, pH7.5, 10% trehalose. |
Bio-activity : | Immobilized Human HVEM, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human BTLA (31-150), Fc Tag, Avi Tag with a linear range of 0.02-0.625 ug/mL. |
Molecular Mass : | The protein has a calculated MW of 42.5 kDa. The protein migrates as 55-66 KDa under reducing condition (SDS-PAGE) due to glycosylation. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Characteristic : | Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | BTLA B and T lymphocyte associated [ Homo sapiens ] |
Official Symbol : | BTLA |
Synonyms : | BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; BTLA1; CD272; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein; FLJ16065; MGC129743; |
Gene ID : | 151888 |
mRNA Refseq : | NM_001085357 |
Protein Refseq : | NP_001078826 |
MIM : | 607925 |
UniProt ID : | Q7Z6A9 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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