Active Recombinant Human BTLA Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: BTLA-567H
• Product Overview: Biotinylated Human BTLA (31-150), Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 31-150 a.a.
• Predicted N Terminal: Lys 31
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arg, 150 mM NaCl, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human HVEM, His Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Human BTLA (31-150), Fc Tag, Avi Tag with a linear range of 0.02-0.625 ug/mL.
• Molecular Mass: The protein has a calculated MW of 42.5 kDa. The protein migrates as 55-66 KDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: BTLA B and T lymphocyte associated [ Homo sapiens ]
• Official Symbol: BTLA
• Synonyms: BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; BTLA1; CD272; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein; FLJ16065; MGC129743
• Gene ID: 151888
• mRNA Refseq: NM_001085357
• Protein Refseq: NP_001078826
• MIM: 607925
• UniProt ID: Q7Z6A9