Active Recombinant Human C1S Protein, His-tagged

• Cat.No.: C1S-597H
• Product Overview: Active Recombinant Human C1S Protein(NP_001725.1) (Met 1-Asp 688) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-688 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is > 7,000 pmoles/min/μg, as measured under the described conditions.
• Molecular Mass: The recombinant human C1s protease consists of 684 amino acids and has a predicted molecular mass of 76.3 kDa. As a result of glycosylation, rhC1s migrates as an approximately 82.06 kDa, 56.59 kDa and 33.36 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 88.5 kDa verified by SEC-MALS(Routinely tested).
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: C1S complement component 1, s subcomponent [ Homo sapiens ]
• Official Symbol: C1S
• Synonyms: C1S; complement component 1, s subcomponent; complement C1s subcomponent; C1 esterase; basic proline-rich peptide IB-1; complement component 1 subcomponent s; FLJ44757
• Gene ID: 716
• mRNA Refseq: NM_001734
• Protein Refseq: NP_001725
• MIM: 120580
• UniProt ID: P09871