Active Recombinant Human CD33 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD33-176HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human CD33 (Accession # AAA51948) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is typically 1-4 μg/mL.
• Molecular Mass: Recombinant Human CD33, Fc Chimera has a calculated MW of 53.4 kDa (monomer). In SDS-PAGE migrates as 67-85 kDa, reducing conditions.
• N-terminal Sequence Analysis: Asp 18
• Purity: > 90 % by SDS-PAGE and analyzed by silver stain
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.

Gene Information

• Gene Name: CD33 CD33 molecule [ Homo sapiens ]
• Official Symbol: CD33
• Synonyms: CD33; CD33 molecule; CD33 antigen (gp67); myeloid cell surface antigen CD33; FLJ00391; p67; sialic acid binding Ig like lectin 3; SIGLEC 3; SIGLEC3; gp67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC-3
• Gene ID: 945
• mRNA Refseq: NM_001082618
• Protein Refseq: NP_001076087
• MIM: 159590
• UniProt ID: P20138