Active Recombinant Human CD4 protein, His-tagged

• Cat.No.: CD4-3167H
• Product Overview: Recombinant Human CD4 protein(Lys26-Trp390), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 26-390 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD4 Protein at 1μg/mL (100 μL/well) can bind CD4 Rabbit mAb with a linear range of 0.006-0.18 ng/mL.
• Molecular Mass: 41.53 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE; ≥ 90 % as determined by HPLC.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
• Publications:
  • Aptamer-Containing Surfaces for Selective Capture of CD4 Expressing Cells (2012)
  • Soluble CD4 inhibits Ebola virus infection by targeting endosomal receptor-binding site (2025)

Gene Information

• Gene Name: CD4 CD4 molecule [ Homo sapiens ]
• Official Symbol: CD4
• Synonyms: CD4; CD4 molecule; CD4 antigen (p55) , T cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; CD4mut
• Gene ID: 920
• mRNA Refseq: NM_000616
• Protein Refseq: NP_000607
• MIM: 186940
• UniProt ID: P01730