Active Recombinant Human CD4 protein, His-tagged

• Cat.No.: CD4-3167H
• Product Overview: Recombinant Human CD4 protein(Lys 26 - Trp 390), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 26-390 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind Human CD4, His Tag with a linear range of 0.8-6 ng/mL.Immobilized Human CD4, His Tag at 1 μg/mL (100 μL/well) can bind Ibalizumab with a linear range of 0.2-13 ng/mL.Immobilized Human CD4, His Tag at 2 μg/mL (100 μL/well) can bind Anti-CD4 with a linear range of 0.4-7 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 41.5 kDa. The protein migrates as 47-54 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Lys26-Trp390
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Aptamer-Containing Surfaces for Selective Capture of CD4 Expressing Cells (2012)

Gene Information

• Gene Name: CD4 CD4 molecule [ Homo sapiens ]
• Official Symbol: CD4
• Synonyms: CD4; CD4 molecule; CD4 antigen (p55) , T cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; CD4mut
• Gene ID: 920
• mRNA Refseq: NM_000616
• Protein Refseq: NP_000607
• MIM: 186940
• UniProt ID: P01730