Active Recombinant Human CD40, Fc Chimera
Cat.No. : | CD40-493H |
Product Overview : | Recombinant Human CD40 encoding the signal peptide and extracellular domain of human CD40 (aa 1-193) was fused to the Fc region of human IgG1 (aa 90-330). The chimeric protein was expressed in modifiedHuman 293 cells. |
Unit | |
Price | |
Qty |
- Specification
- Gene Information
- Related Products
Cat. No. : | CD40-493H |
Description : | CD40 (TNFRSF5, CD40L Receptor) is a type I membrane protein and a member of the TNF receptor family. CD40 is expressed on B cells, monocytes, dendritic cells, endothelial cells, epithelial cells and B cell lymphomas and carcinomas. CD40 activates mulitple signalling pathways and can promote cell survival through up-regulation of the expression of genes encoding anti-apoptopic proteins. |
Source : | Human 293 cells. |
Amino Acid Sequence : | EPPTACREKQYLINSQCCSLCQPGQ KLVSDCTEFTETECLPCGESEFLDT WNRETHCHQHKYCDPNLGLRVQQKG TSETDTICTCEEGWHCTSEACESCV LHRSCSPGFGVKQIATGVSDTICEP CPVGFFSNVSSAFEKCHPWTSCETK DLVVQQAGTNKTDVVCGPQDRLRRS SNTKVDKKVEPKSCDKTHTCPPCPA PELLGGPSVFLFPPKPKDTLMISRT PEVTCVVVDVSHEDPEVKFNWYVDG VEVHNAKTKPREEQYNSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAP IEKTISKAKGQPREPQVYTLPPSRD ELTKNQVSLTCLVKGFYPSDIAVEW ESNGQPENNYKTTPPVLDSDGSFFL YSKLTVDKSRWQQGNVFSCSVMHEA LHNHYTQKSLSLSPGK. |
Molecular Mass : | Under reducing conditions CD40 (173aa) – Fc Chimera migrates as a broad band between 50 and 65 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. |
pI : | CD40 (173aa) – Fc Chimera separates into a number of glycoforms with an observed pI between 5.5 and 9.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. |
% Carbohydrate : | Purified CD40 (173aa) - Fc Chimera consists of 5 to 30% carbohydrate by weight. |
Glycosylation : | CD40 (173aa) – Fc Chimera contains N-linked oligosaccharides and may contain O-linked oligosaccharides. |
Purity : | >95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue. |
Formulation : | When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
Reconstitution : | It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
Storage : | Lyophilized products should be stored at 2 to 8°C. |
Activity : | The ED50of CD40 (173aa) – Fc Chimera is typically 1.0-1.5 ug/ml as measured by its ability to neutralize CD40 ligand mediated proliferation of T47-D cells. |
Gene Name : | CD40 CD40 molecule, TNF receptor superfamily member 5 [ Homo sapiens ] |
Synonyms : | CD40; CD40 molecule, TNF receptor superfamily member 5; p50; Bp50; CDW40; MGC9013; TNFRSF5; CD40 antigen; CD40 type II isoform; CD40L receptor; B cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); nerve growth factor receptor-related B-lymphocyte activation molecule; tumor necrosis factor receptor superfamily, member 5;B-cell surface antigen CD40; OTTHUMP00000031699; OTTHUMP 00000031700 |
Gene ID : | 958 |
mRNA Refseq : | NM_001250 |
Protein Refseq : | NP_001241 |
UniProt ID : | P25942 |
Chromosome Location : | 20q12-q13.2 |
MIM : | 109535 |
Pathway : | ograft rejection; Autoimmune thyroid disease; Cell adhesion molecules (CAMs); Cytokine-cytokine receptor interaction; Primary immunodeficiency; Systemic lupus erythematosus; Toll-like receptor signaling pathway; Signaling in Immune system |
Function : | enzyme binding; receptor activity |
Products Types
◆ Recombinant Protein | ||
CD40-816M | Recombinant Mouse CD40 Protein (Val24-Arg193), His-tagged | +Inquiry |
CD40-629H | Recombinant Human CD40 Protein, Fc-tagged | +Inquiry |
CD40-679H | Recombinant Human CD40 Protein, His-tagged | +Inquiry |
CD40-566R | Recombinant Rhesus Macaque CD40 Protein, His (Fc)-Avi-tagged | +Inquiry |
CD40-928C | Recombinant Canine CD40 Protein (Glu21-Ala194), His-tagged | +Inquiry |
◆ Lysates | ||
CD40-1254CCL | Recombinant Cynomolgus CD40 cell lysate | +Inquiry |
CD40-1831MCL | Recombinant Mouse CD40 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket