Active Recombinant Human CEACAM5 protein, His/Avi-tagged, Biotinylated

• Cat.No.: CEACAM5-052H
• Product Overview: Biotinylated Recombinant Human CEACAM5(Lys35-Ala685) protein, fused to His/Avi tag at the C-terminus, was expressed in CHO cells .

Specification

• Description: CEACAM-5, also known as CEA, CD66e and Psg30, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM family members are highly glycosylated with varying arrangements of IgV-like and IgC-like regions in their extracellular domains (ECD) and can be expressed as transmembrane, glycophosphatidyl inositol (GPI) linked or soluble proteins (1-3). CEACAM-5 consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2, 4, 5). While the mature ECD of human CEACAM-5 shares 26% amino acid identity with mouse CEACAM-5, it remains unclear if these molecules are direct orthologs (6). CEACAM-5, expressed primarily by epithelial cells, functions as a calcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (1, 7). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (8). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is up-regulated in a wide variety of human tumors, promoting tumor cell migration, invasion, adhesion, and metastasis, and has been used as a cancer marker (8, 9). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (10, 11). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (12). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK-mediated lysis (8). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (13, 14). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (14, 15). Our Avi-tag Biotinylated Recombinant Human CEACAM-5 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
• Source: CHO cells
• Species: Human
• Tag: His&Avi
• Predicted N Terminal: Lys35
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human CEACAM5 His-tag Avi-tag is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Galectin-4 binds with an ED50 of 15-120 ng/mL.
• Molecular Mass: 125-155 kDa, under reducing conditions
• Protein length: Lys35-Ala685
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: CEACAM5 carcinoembryonic antigen-related cell adhesion molecule 5 [ Homo sapiens ]
• Official Symbol: CEACAM5
• Synonyms: CEACAM5; carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; meconium antigen 100; DKFZp781M2392
• Gene ID: 1048
• mRNA Refseq: NM_004363
• Protein Refseq: NP_004354
• MIM: 114890
• UniProt ID: P06731

Reviews

03/03/2020

CEACAM5 protein has exhibited remarkable performance in Western Blotting experiments.

08/06/2019

This enhanced visibility and distinctness of the protein bands greatly aided in the accurate analysis and interpretation of my Western Blotting results.

09/23/2018

Its reliability, versatility, and ability to deliver clear and reliable results make it a top choice for researchers in need of a high-quality protein for their experiments.

Q&As

Can CEACAM5 levels be used to detect cancer in its early stages?

CEACAM5 can be a helpful marker, but it is not typically used for early cancer detection.

Are there any limitations to using CEACAM5 as a cancer marker?

CEACAM5 is not specific to a single cancer type and may also be elevated in non-cancerous conditions.

What other medical conditions or diseases can cause an increase in CEACAM5 levels?

Conditions like hepatitis, pancreatitis, and some non-cancerous gastrointestinal diseases can also elevate CEACAM5 levels.

How is CEACAM5 protein used in cancer treatment monitoring?

CEACAM5 levels are monitored to assess the effectiveness of cancer treatment and to detect cancer recurrence.

Can CEACAM5 levels vary between different individuals, and if so, why?

Yes, CEACAM5 levels can vary between individuals due to genetic factors and other individual-specific variables.