Active Recombinant Human CHUK Protein, GST-tagged

• Cat.No.: CHUK-5129H
• Product Overview: Human CHUK (NP_001269.3, 1 a.a. - 745 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq
• Form: Liquid
• Bio-activity: The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization).
  Substrate: I-kappaB-alpha peptide.
  ATP: 100 μM.
• Molecular Mass: 111 kDa
• Purity: 93 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -8 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: CHUK conserved helix-loop-helix ubiquitous kinase [ Homo sapiens ]
• Official Symbol: CHUK
• Synonyms: CHUK; conserved helix-loop-helix ubiquitous kinase; TCF16; inhibitor of nuclear factor kappa-B kinase subunit alpha; IkBKA; IKK alpha; IKK1; IKKA; NFKBIKA; TCF-16; IKK-a kinase; I-kappa-B kinase 1; I-kappa-B kinase-alpha; transcription factor 16; IkB kinase alpha subunit; Nuclear factor NFkappaB inhibitor kinase alpha; IKBKA; IKK-alpha
• Gene ID: 1147
• mRNA Refseq: NM_001278
• Protein Refseq: NP_001269
• MIM: 600664
• UniProt ID: O15111