Active Recombinant Human CSF1

• Cat.No.: CSF1-418H
• Product Overview: Macrophage Colony Stimulating Factor Human Recombinant produced in E.coli is a disulfide linked homodimer, glycosylated, polypeptide chain containing 2 x 149 amino acids and having a total molecular mass of 42 kDa. MCSF is purified by proprietary chromatographic techniques.

Specification

• Description: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants.
• Source: Baculovirus infected Silkworm
• Species: Human
• Form: Sterile Filtered White lyophilized (freeze-dried) powder. The lyophilized protein (1mg/ml) was lyophilized with 20mM phosphate buffer, 1% HSA and 3% manntiol.
• Bio-activity: The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 3ng/ml, corresponding to a specific activity of less than 333,333.33units/mg.
• Molecular Mass: 42 kDa
• AA Sequence: EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQ.
• Purity: Greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
• Storage: Lyophilized Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens (human) ]
• Official Symbol: CSF1
• Synonyms: CSF1; MCSF; CSF-1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; lanimostim; NP_000748.3; EC 2.7.10.1; NP_757349.1; NP_757350.1; NP_757351.1
• Gene ID: 1435
• mRNA Refseq: NM_000757
• Protein Refseq: NP_000748
• MIM: 120420
• UniProt ID: P09603
• Chromosome Location: 1q21-p13
• Pathway: Cytokine-cytokine receptor interaction; Rheumatoid arthritis; Signaling events mediated by TCPTP
• Function: cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding