Recombinant Human/Cynomolgus EFNA3 protein, hFc-tagged

• Cat.No.: EFNA3-160H
• Product Overview: Recombinant Human/Cynomolgus EFNA3 protein(P52797-1)(Gln23-Ser213), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human/Cynomolgus
• Tag: C-hFc
• Protein length: Gln23-Ser213
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human EPHA10, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus EFNA3, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 41.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.