Active Recombinant Human EGFR protein, hFc-tagged, Low Endotoxin

• Cat.No.: EGFR-629H
• Product Overview: Recombinant Human EGFR protein(NP_001333870.1)(Leu25-Ser378), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 25-378 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human EGFR VIII, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-EGFRVIII Antibody, hFc Avi Tag with the EC50 of 23.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 64.58 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533