Active Recombinant Human EPHA2 protein, His&GST-tagged

• Cat.No.: EPHA2-8628H
• Product Overview: Active Recombinant Human EPHA2 protein(P29317) (Leu585-Ile976) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 585-976 aa
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 3mM DTT, pH 8.5, 10% gly.
• Bio-activity: The specific activity was determined to be 50 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
• Molecular Mass: The recombinant human EPHA2 /GST chimera consists of 629 amino acids and has a calculated molecular mass of 72.1 kDa. The recombinant protein migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHA2 EPH receptor A2 [ Homo sapiens ]
• Official Symbol: EPHA2
• Synonyms: EPHA2; EPH receptor A2; ECK, EphA2; ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase; ECK; CTPA; ARCC2; CTPP1
• Gene ID: 1969
• mRNA Refseq: NM_004431
• Protein Refseq: NP_004422
• MIM: 176946
• UniProt ID: P29317