Active Recombinant Human EphA2 protein, His-tagged

• Cat.No.: EPHA2-2027H
• Product Overview: Active Recombinant Human EphA2 protein(P29317) (Met1-Asn534) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-534 aa
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.5.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human EPHA2-His at 10 μg/ml (100 μl/well) can bind human EFNA1-Fch, The EC50 of human EFNA1-Fch is 0.1-0.2 μg/ml.
• Molecular Mass: The recombinant human EPHA2 consists of 522 amino acids and predicts a molecular mass of 57.6 KDa. It migrates as an approximately 65-75 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHA2 EPH receptor A2 [ Homo sapiens ]
• Official Symbol: EPHA2
• Synonyms: EPHA2; EPH receptor A2; ECK, EphA2; ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase; ECK; CTPA; ARCC2; CTPP1
• Gene ID: 1969
• mRNA Refseq: NM_004431
• Protein Refseq: NP_004422
• MIM: 176946
• UniProt ID: P29317