Active Recombinant Human Epidermal Growth Factor, biotinylated

• Cat.No.: EGF-556HB
• Product Overview: Biotinylated Active Recombinant Human Epidermal Growth Factor(NP_001954.2) (Asn971-Arg1023) was expressed and purified with an initial Met at the N-terminus.

Specification

• Tag: Non
• Protein Length: Asn971-Arg1023
• Tag: Non
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. iPSC-derived human vascular organoids (Day 7) were cultured with FGF2, VEGFA, EGF. Red arrows represent vascular organoids. Image taken at 10x magnification. 2. Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.02-0.2ng/ml. 3. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with EGF(10 ng/mL, 30min) using Phospho-EGF Receptor (Tyr1068) rabbit monoclonal Antibody at 1:2000 dilution (upper), or Anti-EGFR Antibody, Rabbit Polyclonal at 1:2000 dilution (lower).
• Molecular Mass: The recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.35 kDa as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: < 5 EU per mg of the protein.
• Purity: ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EGF epidermal growth factor [ Homo sapiens ]
• Official Symbol: EGF
• Synonyms: EGF; epidermal growth factor; epidermal growth factor (beta urogastrone); pro-epidermal growth factor; beta-urogastrone; URG; HOMG4
• Gene ID: 1950
• mRNA Refseq: NM_001178130
• Protein Refseq: NP_001171601
• MIM: 131530
• UniProt ID: P01133