Active Recombinant Human F9 protein, Fc-tagged
Cat.No. : | F9-258H |
Product Overview : | Recombinant Human F9(Thr29-Thr461) fused Fc tag was expressed in HEK293. |
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Description : | Coagulation Factor IX, also known as Christmas Factor, is a secreted by the liver and plays a key role in the activation of the intrinsic clotting cascade. Factor IX consists of a Gla domain, two tandem EGF-like domains, an activation peptide, and an S1 serine protease domain. Mature human Factor IX shares approximately 81% amino acid sequence identity with mouse and rat Factor IX. Alternative splicing generates an additional isoform that lacks the first EGF-like domain. The Gla domain is modified by Vitamin K-dependent gamma-carboxylation and mediates the association of Factor IX with phospholipid bilayers. The activation peptide is removed by Factor XIa mediated cleavage, resulting in heavy and light chains that remain disulfide-linked. Factor IX can also be activated by proteolytic factors in multiple snake venoms. Active Factor IX associates with Factor VIIIa on the platelet surface where it cleaves and activates Factor X, leading to Fibrin deposition and clot formation. The human Factor IX gene is highly polymorphic, and Hemophilia B can be caused by X-linked deficiency of Factor IX activity. |
Source : | HEK293 |
Species : | Human |
Tag : | Fc |
Predicted N Terminal : | Thr29 (pro-mature) and Tyr47 (mature) |
Form : | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
Bio-activity : | Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >30 pmol/min/μg. |
Molecular Mass : | Predicted Molecular Mass: 75 kDa SDS-PAGE: 86-104 kDa, reducing conditions |
Endotoxin : | <0.1 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, -20 to -70 centigrade under sterile conditions after opening. |
Protein length : | Thr29-Thr461 |
Gene Name : | F9 coagulation factor IX [ Homo sapiens ] |
Official Symbol : | F9 |
Synonyms : | F9; coagulation factor IX; Christmas disease; Factor IX; FIX; hemophilia B; plasma thromboplastic component; F9 p22; FIX F9; factor 9; factor IX F9; serine protease; Christmas factor; plasma thromboplastin component; P19; PTC; HEMB; THPH8; MGC129641; MGC129642; |
Gene ID : | 2158 |
mRNA Refseq : | NM_000133 |
Protein Refseq : | NP_000124 |
MIM : | 300746 |
UniProt ID : | P00740 |
Products Types
◆ Recombinant Protein | ||
F9-915R | Recombinant Rabbit F9 Protein, His-tagged | +Inquiry |
F9-1299H | Recombinant Human F9 protein, His-KSI-tagged | +Inquiry |
F9-3626H | Recombinant Human F9 Protein, GST-tagged | +Inquiry |
F9-2502H | Recombinant Human F9 protein(51-120 aa), C-His-tagged | +Inquiry |
F9-914R | Recombinant Rabbit F9 Protein, His-tagged | +Inquiry |
◆ Native Protein | ||
F9-5405M | Native Mouse Coagulation Factor IX | +Inquiry |
F9-266B | Active Native Bovine Factor IX | +Inquiry |
F9-26523H | Active Native Human F9 Protein | +Inquiry |
◆ Lysates | ||
F9-1768MCL | Recombinant Mouse F9 cell lysate | +Inquiry |
F9-1849HCL | Recombinant Human F9 cell lysate | +Inquiry |
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Not For Human Consumption!
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Customer Reviews (3)
Write a reviewThe short half-life makes this product very safe.
This protein has a wide range of applications and can be used in a variety of experiments.
The method of use is simple, which reduces the experimental time and improves the efficiency.
Q&As (6)
Ask a questionThe F9 protein is involved in step IX in the coagulation cascade, which works synergistically with other coagulation factors to promote the normal progress of blood clotting.
Mutations in the F9 gene may lead to loss of protein expression, structural abnormalities, or dysfunction, which in turn affect the normal function of the F9 protein.
F9 protein mutations are primarily associated with hemophilia B, but the specific mutation type and location may have different effects on an individual's clinical presentation.
Mutations in the F9 gene may cause hemophilia B, an inherited coagulation disorder in which patients lack the F9 protein or have low activity and are prone to abnormal bleeding.
The activity of the F9 protein is usually measured by coagulation time or by specific experimental methods (eg, prothrombin time, activated partial thromboplastin time, etc.).
F9 protein is a single-chain glycoprotein consisting of 415 amino acids. It contains a signaling peptide and multiple functional regions in which the Gla domain at the C-terminal binds to calcium ions and the EGF-like domain at the N-terminus participates in receptor binding and activation.
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