Active Recombinant Human FGF10 Protein (187 aa), N-His-tagged

Cat.No. : FGF10-422F
Product Overview : Recombinant human Fibroblast Growth Factor-10 (rhFGF-10) with N-terminal His-tag produced in E. coli is a single non-glycosylated polypeptide chain containing 187 amino acids. A fully biologically active molecule, rhFGF-10 has a molecular mass of 21.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
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Description : Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares the homolog and receptor FGFR2-IIIb with KGF. However, unlike KGF which induces the proliferation and differentiation of various epithelial cells, FGF-10 is an essential factor for the budding and branching morphogenesis during the multi-organ development via the instructive mesenchymal-epithelial interactions. FGF-10 is crucial for lung and limb development, and is regulated by Shh during early development.
Source : E. coli
Species : Human
Tag : N-His
Form : Sterile Filtered White lyophilized (freeze-dried) powder.
Bio-activity : ED50 < 20 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0 × 10^4 units/mg.
Molecular Mass : 21.4 kDa, observed by reducing SDS-PAGE.
Protein Length : 187
AA Sequence : MNHKVHHHHHHMDDDDKMLGQDMVS PEATNSSSSSFSSPSSAGRHVRSYN HLQGDVRWRKLFSFTKYFLKIEKNG KVSGTKKENCPYSILEITSVEIGVV AVKAINSNYYLAMNKKGKLYGSKEF NNDCKLKERIEENGYNTYASFNWQH NGRQMYVALNGKGAPRRGQKTRRKN TSAHFLPMVVHS
Endotoxin : < 0.2 EU/μg, determined by LAL method.
Purity : > 95% by SDS-PAGE analysis.
Storage : Lyophilized recombinant human Fibroblast Growth Factor-10 (rhFGF-10) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhFGF-10 should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
Storage Buffer : Lyophilized after extensive dialysis against PBS.
Reconstitution : Reconstituted in ddH2O at 100 μg/mL.
Gene Name : FGF10 fibroblast growth factor 10 [ Homo sapiens ]
Official Symbol : FGF10
Synonyms : FGF10; fibroblast growth factor 10; FGF-10; keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria;
Gene ID : 2255
mRNA Refseq : NM_004465
Protein Refseq : NP_004456
MIM : 602115
UniProt ID : O15520

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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