| Description : |
Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 0.25 ng/mL, measured by the cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 4 × 10^6 units/mg. |
| Molecular Mass : |
16.4 kDa, observed by reducing SDS-PAGE. |
| Protein Length : |
146 |
| AA Sequence : |
PALPEDGGSGAFPPGHFKDPKRLYC KNGGFFLRIHPDGRVDGVREKSDPH IKLQLQAEERGVVSIKGVCANRYLA MKEDGRLLASKCVTDECFFFERLES NNYNTYRSRKYTSWYVALKRTGQYK LGSKTGPGQKAILFLPMSAKS |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% by SDS-PAGE analysis. |
| Storage : |
Lyophilized recombinant human Fibroblast Growth Factor-basic (146 a.a.) (rhFGF-basic) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhFGF-basic remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O at 50 μg/mL. |
