Active Recombinant Human FLT1 Protein, His-Avi-tagged, Biotinylated

• Cat.No.: FLT1-3810H
• Product Overview: Biotinylated Human VEGF R1, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Ser 27 - Asn 756 (Accession # P17948-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Predicted N Terminal: Ser 27
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human VEGF165, Tag Free at 2 ug/mL (100 uL/well) can bind Biotinylated Human VEGF R1, His Tag, Avi Tag with a linear range of 1-31 ug/mL. Immobilized Human PLGF, His Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human VEGF R1, His Tag, Avi Tag with a linear range of 2-20 ug/mL.
• Molecular Mass: The protein has a calculated MW of 85.9 kDa. The protein migrates as 106-125 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE..
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: FLT1 fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) [ Homo sapiens ]
• Official Symbol: FLT1
• Synonyms: FLT1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); FLT; vascular endothelial growth factor receptor 1; VEGFR1; FLT-1; VEGFR-1; fms-like tyrosine kinase 1; tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor
• Gene ID: 2321
• mRNA Refseq: NM_001159920
• Protein Refseq: NP_001153392
• MIM: 165070
• UniProt ID: P17948