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Active Recombinant Human FLT1 Protein, His-Avi-tagged, Biotinylated

Cat.No. : FLT1-3810H
Product Overview : Biotinylated Human VEGF R1, His Tag, Avi Tag is expressed from HEK293 cells. It contains AA Ser 27 - Asn 756 (Accession # P17948-1). This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
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Source : HEK293
Species : Human
Tag : His-Avi
Predicted N Terminal : Ser 27
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity : Immobilized Human VEGF165, Tag Free at 2 ug/mL (100 uL/well) can bind Biotinylated Human VEGF R1, His Tag, Avi Tag with a linear range of 1-31 ug/mL. Immobilized Human PLGF, His Tag at 1 ug/mL (100 uL/well) can bind Biotinylated Human VEGF R1, His Tag, Avi Tag with a linear range of 2-20 ug/mL.
Molecular Mass : The protein has a calculated MW of 85.9 kDa. The protein migrates as 106-125 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95% as determined by SDS-PAGE..
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : FLT1 fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) [ Homo sapiens ]
Official Symbol : FLT1
Synonyms : FLT1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); FLT; vascular endothelial growth factor receptor 1; VEGFR1; FLT-1; VEGFR-1; fms-like tyrosine kinase 1; tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor;
Gene ID : 2321
mRNA Refseq : NM_001159920
Protein Refseq : NP_001153392
MIM : 165070
UniProt ID : P17948

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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