Active Recombinant Human GDF7 Protein (Lys352-Arg450), C-His tagged, Animal-free, Carrier-free

• Cat.No.: GDF7-35H
• Product Overview: Recombinant Human GDF7 Protein (Lys352-Arg450) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Bone Morphogenetic Protein-12 (BMP-12), known as Growth differentiation factor 7 (GDF7), is an extracellular multifunctional cytokine that is also a member of the TGFβ family. BMP-12 can bind with the TGFβ receptor and trigger SMAD protein signal transduction. BMP-12 is in a signaling center called the roof plate in the embryo's developing nervous system, where it sends signals to induce the generation of sensory neurons.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 2 μg/mL.
• Molecular Mass: The protein has a calculated MW of 14.95 kDa.
  The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Lys352-Arg450
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: GDF7 growth differentiation factor 7 [ Homo sapiens (human) ]
• Official Symbol: GDF7
• Synonyms: GDF7; growth differentiation factor 7; growth/differentiation factor 7; BMP12; GDF-7
• Gene ID: 151449
• mRNA Refseq: NM_182828
• Protein Refseq: NP_878248
• MIM: 604651
• UniProt ID: Q7Z4P5