Active Recombinant Human HGF protein

Species :	Human
Source :	CHO
Tag :	Non
Protein Length :	1-728 aa
Form :	Lyophilized from sterile PBS, pH 7.4.
Bio-activity :	Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 for this effect is typically 2-10 ng/mL.
Molecular Mass :	The secreted recombinant human HGF consists of 697 amino acids after cleavage of the signal peptide and has a predicted molecular mass of 79.7 kDa. The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. As a result of glycosylation, it migrates with the apparent molecular mass of 90, 60 and 34 kDa corresponding to the single chain, α chain and β chain respectively in SDS-PAGE under reducing conditions.
Endotoxin :	< 1.0 EU per μg protein as determined by the LAL method.
Purity :	> 93 % as determined by SDS-PAGE
Storage :	Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution :	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Name	HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ]
Official Symbol	HGF
Synonyms	HGF; hepatocyte growth factor (hepapoietin A; scatter factor); deafness, autosomal recessive 39 , DFNB39; hepatocyte growth factor; F TCF; fibroblast derived tumor cytotoxic factor; hepatopoietin A; HGFB; HPTA; lung fibroblast derived mitogen; scatter factor; SF; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor; F-TCF; DFNB39;
Gene ID	3082
mRNA Refseq	NM_000601
Protein Refseq	NP_000592
MIM	142409
UniProt ID	P14210