Active Recombinant Human HGF protein

• Cat.No.: HGF-8535H
• Product Overview: Active Recombinant Human HGF protein(NP_000592.3)(Met 1-Ser 728) was expressed and purified in CHO.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Protein Length: 1-728 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 for this effect is typically 2-10 ng/mL.
• Molecular Mass: The secreted recombinant human HGF consists of 697 amino acids after cleavage of the signal peptide and has a predicted molecular mass of 79.7 kDa. The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. As a result of glycosylation, it migrates with the apparent molecular mass of 90, 60 and 34 kDa corresponding to the single chain, α chain and β chain respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 93 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ]
• Official Symbol: HGF
• Synonyms: HGF; hepatocyte growth factor (hepapoietin A; scatter factor); deafness, autosomal recessive 39 , DFNB39; hepatocyte growth factor; F TCF; fibroblast derived tumor cytotoxic factor; hepatopoietin A; HGFB; HPTA; lung fibroblast derived mitogen; scatter factor; SF; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor; F-TCF; DFNB39
• Gene ID: 3082
• mRNA Refseq: NM_000601
• Protein Refseq: NP_000592
• MIM: 142409
• UniProt ID: P14210