| Cat.No. : |
IL10RA-051H
Optional Service: Optional requirements on this protein
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| Product Overview : |
Biotinylated Recombinant Human IL10RA(His22-Asn235) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells . |
| Description : |
Interleukin-10 Receptor alpha (IL-10 RA), also known as IL-10 R1, is a 90-110 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1).
IL-10 RA is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer (2-
5). Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6). IL-10 Rα is the ligand specific subunit of the IL-10 receptor complex.
Noncovalent dimers of IL-10 bind to IL-10 Rα, resulting in the recruitment of IL-10 Rβ (6-8). IL-10 Rβ is a ubiquitously expressed transmembrane protein that does not
bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 Rβ also associates with IL-20 Rα, IL-22 Rα, or IL-28 Rα to
form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (1). Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by
activation of TLR2, 4, or 9, enabling strengthened immune responses during infection (10). Polymorphisms of human IL-10 Rα may limit viral immune evasion by
retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature human IL-10 Rα consists of a 214 amino
acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain (12). Within the ECD, human IL-10 Rα shares 59% aa
sequence identity with mouse and rat IL-10 Rα |
| Source : |
CHO cells |
| Species : |
Human |
| Tag : |
Fc/Avi |
| Predicted N Terminal : |
His22 |
| Form : |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Bio-activity : |
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.
Measured by its binding ability in a functional ELISA. When Recombinant Human IL10 is coated at 2 µg/mL (100 μL/well), Biotinylated Recombinant Human IL10RA Fc Chimera Avi-tag binds with 20-100 ng/mL. |
| Molecular Mass : |
74-85 kDa, under reducing conditions |
| Protein length : |
His22-Asn235 |
| Endotoxin : |
<0.10 EU per 1 μg of the protein by the LAL method. |
| Purity : |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Applications : |
Bioactivity |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution : |
Reconstitute at 500 μg/mL in PBS. |
| SDS-PAGE : |
2 μg/lane Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining.
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| Binding Activity : |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
