Active Recombinant Human IL10RA protein, Fc/Avi-tagged, Biotinylated

• Cat.No.: IL10RA-051H
• Product Overview: Biotinylated Recombinant Human IL10RA(His22-Asn235) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells .

Specification

• Description: Interleukin-10 Receptor alpha (IL-10 RA), also known as IL-10 R1, is a 90-110 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1).
  IL-10 RA is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer (2-
  5). Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6). IL-10 Rα is the ligand specific subunit of the IL-10 receptor complex.
  Noncovalent dimers of IL-10 bind to IL-10 Rα, resulting in the recruitment of IL-10 Rβ (6-8). IL-10 Rβ is a ubiquitously expressed transmembrane protein that does not
  bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 Rβ also associates with IL-20 Rα, IL-22 Rα, or IL-28 Rα to
  form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (1). Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by
  activation of TLR2, 4, or 9, enabling strengthened immune responses during infection (10). Polymorphisms of human IL-10 Rα may limit viral immune evasion by
  retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature human IL-10 Rα consists of a 214 amino
  acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain (12). Within the ECD, human IL-10 Rα shares 59% aa
  sequence identity with mouse and rat IL-10 Rα
• Source: CHO cells
• Species: Human
• Tag: Fc/Avi
• Predicted N Terminal: His22
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.
  Measured by its binding ability in a functional ELISA. When Recombinant Human IL10 is coated at 2 µg/mL (100 μL/well), Biotinylated Recombinant Human IL10RA Fc Chimera Avi-tag binds with 20-100 ng/mL.
• Molecular Mass: 74-85 kDa, under reducing conditions
• Protein length: His22-Asn235
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: IL10RA interleukin 10 receptor, alpha [ Homo sapiens ]
• Official Symbol: IL10RA
• Synonyms: IL10RA; interleukin 10 receptor, alpha; IL10R; interleukin-10 receptor subunit alpha; CD210; CD210a; CDW210A; HIL 10R; IL-10RA; IL-10R subunit 1; IL-10R subunit alpha; IL-10 receptor subunit alpha; interleukin-10 receptor subunit 1; interleukin-10 receptor alpha chain; HIL-10R; IL-10R1
• Gene ID: 3587
• mRNA Refseq: NM_001558
• Protein Refseq: NP_001549
• MIM: 146933
• UniProt ID: Q13651