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Active Recombinant Human IL10RA protein, Fc/Avi-tagged, Biotinylated
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Cat.No. : | IL10RA-051H Optional Service: Optional requirements on this protein |
Product Overview : | Biotinylated Recombinant Human IL10RA(His22-Asn235) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells . |
Description : | Interleukin-10 Receptor alpha (IL-10 RA), also known as IL-10 R1, is a 90-110 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1). IL-10 RA is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer (2- 5). Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6). IL-10 Rα is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 Rα, resulting in the recruitment of IL-10 Rβ (6-8). IL-10 Rβ is a ubiquitously expressed transmembrane protein that does not bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 Rβ also associates with IL-20 Rα, IL-22 Rα, or IL-28 Rα to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (1). Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection (10). Polymorphisms of human IL-10 Rα may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature human IL-10 Rα consists of a 214 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain (12). Within the ECD, human IL-10 Rα shares 59% aa sequence identity with mouse and rat IL-10 Rα |
Source : | CHO cells |
Species : | Human |
Tag : | Fc/Avi |
Predicted N Terminal : | His22 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Bio-activity : | The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human IL10 is coated at 2 µg/mL (100 μL/well), Biotinylated Recombinant Human IL10RA Fc Chimera Avi-tag binds with 20-100 ng/mL. |
Molecular Mass : | 74-85 kDa, under reducing conditions |
Protein length : | His22-Asn235 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Applications : | Bioactivity |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 500 μg/mL in PBS. |
SDS-PAGE : |
![]() 2 μg/lane Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining. |
Binding Activity : |
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Gene Name : | IL10RA interleukin 10 receptor, alpha [ Homo sapiens ] |
Official Symbol : | IL10RA |
Synonyms : | IL10RA; interleukin 10 receptor, alpha; IL10R; interleukin-10 receptor subunit alpha; CD210; CD210a; CDW210A; HIL 10R; IL-10RA; IL-10R subunit 1; IL-10R subunit alpha; IL-10 receptor subunit alpha; interleukin-10 receptor subunit 1; interleukin-10 receptor alpha chain; HIL-10R; IL-10R1; |
Gene ID : | 3587 |
mRNA Refseq : | NM_001558 |
Protein Refseq : | NP_001549 |
MIM : | 146933 |
UniProt ID : | Q13651 |
◆ Recombinant Protein
Il10ra-1658M | Recombinant Mouse Il10ra Protein, His-tagged | +Inquiry |
IL10RA-001H | Recombinant Human IL10RA Protein | +Inquiry |
IL10RA-002H | Recombinant Human IL10RA Protein | +Inquiry |
Il10ra-1234M | Recombinant Mouse Il10ra Protein, MYC/DDK-tagged | +Inquiry |
IL10RA-988R | Recombinant Rhesus IL10RA Protein (Thr9-Asn245), His-tagged | +Inquiry |
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◆ Lysates
IL10RA-1185CCL | Recombinant Cynomolgus IL10RA cell lysate | +Inquiry |
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IL10IL10RAIL10RBIL11IL11RAIl11ra1IL12IL12AIL12BIL12RB1IL13IL13RA1IL13RA2IL15IL15RAIL16IL17AIL17BIl17cIL17DIl17fIL17RAIL17RDIL18IL18BPIL18R1IL18RAPIl19Il1aIl1bIL1F10IL1R1Il1r2IL1RAPIL1RAPL1IL1RAPL2IL1RL1IL1RL2Il1rnIL2Il20IL20RAIL21IL21RIL22IL22BPIL22RA1IL22RA2IL23AIL24Il25IL27IL27RAIL28AIl28bIL29IL2RAIL2RBIl2rgIL3IL31IL32Il33Il34IL3RAIL4IL4RIl4raIl5IL5RAIl6IL6RIl6raIL6STIl7IL7RIL8IL9IL36AIl12rb2IL17RBIL23RIL26IL31RAIL36BIL36GIL36RNIL37IL9RIL17RCIL17REIL28RAIL4I1IL20RBIL17RELIl11ra2IL27bIL7RAIl23a&Il12b
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