Active Recombinant Human IL12B Protein
| Cat.No. : | IL12B-181I |
| Product Overview : | Recombinant Human IL12B Protein without tag was expressed in HEK 293. |
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| Description : | Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the mouse system. |
| Source : | HEK 293 |
| Species : | Human |
| Form : | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : | EC50 < 2 ng/mL, measured by the induction of IFN-γ from NK cells co-stimulated with IL-18, corresponding to a specific activity of > 1 × 10^6 units/mg. |
| Molecular Mass : | Approximately 75 kDa, consisting of a 306 amino acid residue p40 subunit and a 197 amino acid residue p35 subunit. |
| AA Sequence : | p35:RNLPVATPDPGMFPCLHHSQNLLRA VSNMLQKARQTLEFYPCTSEEIDHE DITKDKTSTVEACLPLELTKNESCL NSRETSFITNGSCLASRKTSFMMAL CLSSIYEDLKMYQVEFKTMNAKLLM DPKRQIFLDQNMLAVIDELMQALNF NSETVPQKSSLEEPDFYKTKIKLCI LLHAFRIRAVTIDRVMSYLNAS p40:IWELKKDVYVVELDWYPDAPGEMVV LTCDTPEEDGITWTLDQSSEVLGSG KTLTIQVKEFGDAGQYTCHKGGEVL SHSLLLLHKKEDGIWSTDILKDQKE PKNKTFLRCEAKNYSGRFTCWWLTT ISTDLTFSVKSSRGSSDPQGVTCGA ATLSAERVRGDNKEYEYSVECQEDS ACPAAEESLPIEVMVDAVHKLKYEN YTSSFFIRDIIKPDPPKNLQLKPLK NSRQVEVSWEYPDTWSTPHSYFSLT FCVQVQGKSKREKKDRVFTDKTSAT VICRKNASISVRAQDRYYSSSWSEW ASVPCS |
| Endotoxin : | < 0.2 EU/μg, determined by LAL method. |
| Purity : | > 95% as analyzed by SDS-PAGE and HPLC. |
| Storage : | Lyophilized recombinant Human Interleukin 12(IL-12) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rh-IL12 should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : | Lyophilized after extensive dialysis against PBS. |
| Reconstitution : | Reconstituted in ddH2O or PBS at 100 μg/mL. |
| Gene Name : | IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ] |
| Official Symbol : | IL12B |
| Synonyms : | IL12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; cytotoxic lymphocyte maturation factor 2; p40; IL 12B; IL12; subunit p40; interleukin 12; interleukin 12 beta chain; natural killer cell stimulatory factor; 40 kD subunit; natural killer cell stimulatory factor 2; NKSF; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor, 40 kD subunit; IL-12B; |
| Gene ID : | 3593 |
| mRNA Refseq : | NM_002187 |
| Protein Refseq : | NP_002178 |
| MIM : | 161561 |
| UniProt ID : | P29460 |
Products Types
| ◆ Lysates | ||
| IL12B-1000MCL | Recombinant Marmoset IL12B cell lysate | +Inquiry |
| IL12B-2731HCL | Recombinant Human IL12B cell lysate | +Inquiry |
| IL12B-1197RCL | Recombinant Rat IL12B cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
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04/08/2020
Good for cytokine assays.
01/06/2020
Effective in Th2 cell differentiation.
01/12/2019
High yield in expression.
Q&As (10)
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Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation?
05/12/2021
ChIP-seq identifies regulatory elements controlling IL12B gene expression.
Can you elaborate on the use of reporter assays to monitor IL12B promoter activity?
05/02/2021
Reporter assays monitor IL12B promoter activity under varying conditions.
What methods evaluate IL12B's response to different pathogenic stimuli in primary cells?
10/30/2020
Primary cell stimulation assays gauge IL12B response to pathogens.
How does siRNA knockdown of IL12B elucidate its role in immune cell activation?
05/25/2020
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
How does recombinant protein expression and purification aid in IL12B functional studies?
04/30/2020
Recombinant protein expression enables functional IL12B studies in vitro.
How does co-immunoprecipitation validate IL12B's association with signaling partners?
04/12/2018
Co-IP validates IL12B's binding to specific signaling partners.
What techniques are employed to analyze the impact of IL12B genetic variants on protein function?
12/03/2017
Genetic variant analysis reveals impacts on IL12B protein function.
How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets?
10/31/2017
Flow cytometry profiles IL12B expression across diverse immune cell types.
What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation?
09/29/2017
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
What are the methods to assess IL12B's protein-protein interactions in a cellular context?
08/22/2017
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
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