Active Recombinant Human IL15

• Cat.No.: IL15-25H
• Product Overview: Recombinant Human Interleukin-15/IL-15 produced in E. coli is a single non-glycosylated polypeptide chain containing 114 amino acids with a molecular mass of 12.5 kD.

Specification

• Description: Human Interleukin 15 (IL-15) is a cytokine that regulates T cell and natural killer cell activation and proliferation. IL-15 binds to the alpha subunit of the IL15 receptor (IL-15RA) with high affinity. IL-15 also binds to the beta and gamma chains of the IL-2 receptor, but not the alpha subunit of the IL2 receptor. IL-15 is structurally and functionally related to IL-2. Both cytokines share some subunits of receptors, allowing them to compete for and negatively regulate each other"s activity. The number of CD8+ memory T cells is controlled by a balance between IL-15 and IL-2. Despite their many overlapping functional properties, IL-2 and IL-15 are, in fact, quite distinct players in the immune system. IL-15 is constitutively expressed by a wide variety of cell types and tissues, including monocytes, macrophages and DCs. Mature Human IL-15 shares 70% amino acid sequence identity with Mouse and Rat IL-15.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.0.
• Bio-activity: ED50 is less than 0.5 ng/ml as determined by the dose dependent stimulation of mouse CTLL-2 cell proliferation. Specific Activity of 2.0 x 10^6 IU/mg.
• Endotoxin: Less than 0.1 EU/μg.
• Purity: Greater than 95% as determined by RP-HPLC and reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at< -20°c, though stable at room temperature for 3>Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at< -20°c for 3>
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in 1X PBS.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: IL15 interleukin 15 [ Homo sapiens ]
• Official Symbol: IL15
• Synonyms: IL15; interleukin 15; interleukin-15; IL 15; MGC9721; IL-15
• Gene ID: 3600
• mRNA Refseq: NM_000585
• Protein Refseq: NP_000576
• MIM: 600554
• UniProt ID: P40933
• Chromosome Location: 4q31
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem
• Function: cytokine activity; cytokine receptor binding; protein binding

Reviews

04/19/2018

Mapping protein- interactions in DNA transcription for gene regulation.

02/20/2018

Suitable for cytotoxicity assays.

10/22/2017

Effective in NK cell activation.

Q&As

What techniques differentiate IL15 and IL2 receptor binding kinetics?

Techniques discern IL15 and IL2 receptor binding kinetics.

What experimental methods study IL15's involvement in autoimmune diseases?

Methods explore IL15's role in autoimmune disease mechanisms.

What are the applications of IL15 knockout models in deciphering its functions?

IL15 knockout models provide insights into its diverse functions.

How do you analyze IL15's impact on memory T cell differentiation?

IL15's influence on memory T cell differentiation investigated.

How does immunofluorescence microscopy visualize IL15 localization in cells?

Immunofluorescence microscopy visualizes IL15 subcellular localization.

What approaches validate IL15's role in inflammatory responses?

Approaches validate IL15's involvement in inflammatory responses.

Could you explain the role of IL15 in NK cell cytotoxicity assays?

IL15's role in NK cell cytotoxicity analyzed using assays.

Can you detail the use of ELISA in quantifying IL15 secretion?

ELISA measures IL15 secretion accurately.

How is IL15's effect on regulatory T cells quantified using qPCR?

qPCR quantifies IL15's impact on regulatory T cells.

How does flow cytometry assess IL15-induced T-cell proliferation?

Flow cytometry quantifies IL15-driven T-cell proliferation.