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Active Recombinant Human IL15, HIgG1 Fc-tagged

Cat.No. : IL15-006H
Product Overview : The extracellular domain of human IL-15(NP_751914.1)(Asn49-Ser162) including mutations at Q149D and Q156D is fused to the N-terminus of the Fc region of human IgG1 was expressed in CHO cell.
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Description : The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other"s activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported
Source : CHO cells
Species : Human
Tag : HIgG1 Fc-tagged
Form : Lyophilized from 0.2μm-filtered solution in PBS.
Bio-activity : This mutant IL-15/Fc fusion protein specifically binds to the IL-15R, competitively inhibits IL-15-triggered cell proliferation, promotes transplant tolerance, does not activate the STATsignaling pathway, and exerts a prolonged circulating half-life cause
Molecular Mass : 41 KDa (monomer)
AA Sequence : Asn49-Ser162
Purity : >98%, by SDS-PAGE under reducing conditions.
Stability : Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Reconstitution : Reconstitute at 100μg/ml in sterile PBS.
Warning : Avoid freeze/thaw cycles.
Gene Name : IL15 interleukin 15 [ Homo sapiens ]
Official Symbol : IL15
Synonyms : IL15; interleukin 15; interleukin-15; IL 15; MGC9721; IL-15;
Gene ID : 3600
mRNA Refseq : NM_000585
Protein Refseq : NP_000576
MIM : 600554
UniProt ID : P40933
Chromosome Location : 4q31
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem;
Function : cytokine activity; cytokine receptor binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What techniques differentiate IL15 and IL2 receptor binding kinetics? 10/22/2022

Techniques discern IL15 and IL2 receptor binding kinetics.

What experimental methods study IL15's involvement in autoimmune diseases? 06/23/2022

Methods explore IL15's role in autoimmune disease mechanisms.

What are the applications of IL15 knockout models in deciphering its functions? 04/26/2022

IL15 knockout models provide insights into its diverse functions.

How do you analyze IL15's impact on memory T cell differentiation? 09/19/2021

IL15's influence on memory T cell differentiation investigated.

How does immunofluorescence microscopy visualize IL15 localization in cells? 07/26/2021

Immunofluorescence microscopy visualizes IL15 subcellular localization.

What approaches validate IL15's role in inflammatory responses? 02/04/2021

Approaches validate IL15's involvement in inflammatory responses.

Could you explain the role of IL15 in NK cell cytotoxicity assays? 10/26/2019

IL15's role in NK cell cytotoxicity analyzed using assays.

Can you detail the use of ELISA in quantifying IL15 secretion? 02/03/2018

ELISA measures IL15 secretion accurately.

How is IL15's effect on regulatory T cells quantified using qPCR? 06/08/2017

qPCR quantifies IL15's impact on regulatory T cells.

How does flow cytometry assess IL15-induced T-cell proliferation? 05/26/2017

Flow cytometry quantifies IL15-driven T-cell proliferation.

Customer Reviews (3)

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Reviews
04/19/2018

    Mapping protein- interactions in DNA transcription for gene regulation.

    02/20/2018

      Suitable for cytotoxicity assays.

      10/22/2017

        Effective in NK cell activation.

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